biotin sba (Vector Laboratories)
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Vector Laboratories
biotin sba
Biotin Sba, supplied by Vector Laboratories, used in various techniques. Bioz Stars score: 93/100, based on 53 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/B-1015-5/Biotinylated+Soybean+Agglutinin+(SBA)/pm42878060-206-14-15
Average 93 stars, based on 53 article reviews
Biotin Sba, supplied by Vector Laboratories, used in various techniques. Bioz Stars score: 93/100, based on 53 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/B-1015-5/Biotinylated+Soybean+Agglutinin+(SBA)/pm42878060-206-14-15
Average 93 stars, based on 53 article reviews
biotin sba - by Bioz Stars,
2026-11
93/100 stars
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Binding Assay:Article Title: Ligand-specific tuning of CLEC10A signalling strength and dendritic cell responses through engagement of different GalNAc-containing glycan structures. Article Snippet: See the T erm s and C onditions (https://onlinelibrary.w iley.com /term s-and-conditions) on W iley O nline L ibrary for rules of use; O A articles are governed by the applicable C reative C om m ons L icense acetic acid (Fluka, Charlotte, NC, USA/Sigma-Aldrich) in DMSO and incubated for 2 h at 37° C. Subsequently, glycodendrimers were separated from unbound oligosaccharides on PD10 desalting columns (Sigma-Aldrich) according to the manufacturer’s protocol and lyophilised at least five times to improve purity. .. To determine the lectin binding, glycodendrimers were serially diluted in 0.2 M NaHCO3 (Sigma-Aldrich; pH 9.2) and coated onto NUNC Maxisorp plates (Thermo Fisher, Waltham, MA, USA) overnight at 4° C. Plates were blocked with TSM (20 mM Tris, 150 mM NaCl, 2 mM MgCl2 (all Sigma-Aldrich) and 1 mM CaCl2 (VWR chemicals, Radnor, PA, USA)), containing 0.5% bovine serum albumin (BSA fraction V, Roche, Basel, Switzerland) for 30 min at 37° C. Afterwards, 0.5 lg mL 1 CLEC10A-Fc, CLEC10A H259T-Fc, DC-SIGN-Fc (all produced in house), or biotinylated Helix pomatia agglutinin (HPA; Sigma-Aldrich) or 1 lg mL 1 Produced:Article Title: Ligand-specific tuning of CLEC10A signalling strength and dendritic cell responses through engagement of different GalNAc-containing glycan structures. Article Snippet: See the T erm s and C onditions (https://onlinelibrary.w iley.com /term s-and-conditions) on W iley O nline L ibrary for rules of use; O A articles are governed by the applicable C reative C om m ons L icense acetic acid (Fluka, Charlotte, NC, USA/Sigma-Aldrich) in DMSO and incubated for 2 h at 37° C. Subsequently, glycodendrimers were separated from unbound oligosaccharides on PD10 desalting columns (Sigma-Aldrich) according to the manufacturer’s protocol and lyophilised at least five times to improve purity. .. To determine the lectin binding, glycodendrimers were serially diluted in 0.2 M NaHCO3 (Sigma-Aldrich; pH 9.2) and coated onto NUNC Maxisorp plates (Thermo Fisher, Waltham, MA, USA) overnight at 4° C. Plates were blocked with TSM (20 mM Tris, 150 mM NaCl, 2 mM MgCl2 (all Sigma-Aldrich) and 1 mM CaCl2 (VWR chemicals, Radnor, PA, USA)), containing 0.5% bovine serum albumin (BSA fraction V, Roche, Basel, Switzerland) for 30 min at 37° C. Afterwards, 0.5 lg mL 1 CLEC10A-Fc, CLEC10A H259T-Fc, DC-SIGN-Fc (all produced in house), or biotinylated Helix pomatia agglutinin (HPA; Sigma-Aldrich) or 1 lg mL 1 Whole Genome Amplification:Article Title: Glycopolymer-Functionalized Gold Nanoparticles for the Detection of Western Diamondback Rattlesnake ( Crotalus atrox ) Venom Article Snippet: High-performance liquid chromatography (HPLC)-grade acetonitrile (≥99.8%), glucose (lab-reagent grade), hexane fraction from petrol (lab-reagent grade), DCM (99% lab reagent grade), sodium hydrogen carbonate (≥99%), ethyl acetate (≥99.7%, analytical reagent grade), sodium chloride (≥99.5%), calcium chloride, 40–60 petroleum ether (lab reagent grade), hydrochloric acid (∼37%, analytical grade), glacial acetic acid (analytical grade), and magnesium sulfate (reagent grade) were purchased from Thermo Fisher Scientific. .. Article Title: Glycopolymer-Functionalized Gold Nanoparticles for the Detection of Western Diamondback Rattlesnake ( Crotalus atrox ) Venom. Article Snippet: High-performance liquid chromatography (HPLC)-grade acetonitrile (≥99.8%), glucose (lab-reagent grade), hexane fraction from petrol (lab-reagent grade), DCM (99% lab reagent grade), sodium hydrogen carbonate (≥99%), ethyl acetate (≥99.7%, analytical reagent grade), sodium chloride (≥99.5%), calcium chloride, 40−60 petroleum ether (lab reagent grade), hydrochloric acid (∼37%, analytical grade), glacial acetic acid (analytical grade), and magnesium sulfate (reagent grade) were purchased from Thermo Fisher Scientific. .. Article Title: Lipopolysaccharide Detection with Glycan-Specific Lectins—a Nonspecific Binding Approach Applied to Surface Plasmon Resonance Article Snippet: Phosphate buffer solution pH 7.4 (1X PBS) was purchased from Corning (Corning, US). .. Biotinylated lectins Concanavalin (ConA), Enzyme-linked Immunosorbent Assay:Article Title: Aberrant glycosylation patterns as potential biomarkers for diagnosis and disease progression in bullous pemphigoid Article Snippet: .. Briefly, during Lectin ELISA of serum and blister fluid, biotinylated lectins were diluted with 1% BSA to the following concentrations: HPA-L (Vector, B-1115) 5μg/mL, SNA (Vector, B-1305) 0.5μg/mL, PSA (Vector, B-1055) 0.5μg/mL, Concentration Assay:Article Title: Graph attention network-driven single-cell omics analysis reveals how B4GALNT1-mediated glycosylation remodelling of the ferroptosis-pyroptosis network promotes drug resistance in hepatocellular carcinoma. Article Snippet: .. BIE et al. 9 of 28 Biotin-labelled lectins, including Biotin-VVA (Vector Laboratories, B-1231, USA), Incubation:Article Title: Graph attention network-driven single-cell omics analysis reveals how B4GALNT1-mediated glycosylation remodelling of the ferroptosis-pyroptosis network promotes drug resistance in hepatocellular carcinoma. Article Snippet: .. BIE et al. 9 of 28 Biotin-labelled lectins, including Biotin-VVA (Vector Laboratories, B-1231, USA), |
